ATP-dependent recombinant DNA ligase broadly utilized for ligase-mediated mutation detection methods (OLA, LCR, LDR, LAR, RCA), DNA sequencing (also NGS), molecular cloning, site-directed mutagenesis and nick repair in duplex DNA, RNA or DNA/RNA hybrids. It accepts nicked dsDNA, cohesive ends and fully base-paired (blunt end) substrates for in vitro ligation reactions. It is free of contaminated nucleases and has very reduced amount of host DNA.
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