Highly active Taq DNA polymerase without 5'to 3' exonuclease activity. Stoffel Fragment works optimally at a broader range of MgCl2 concentration (2-10 mM) as compared to Taq polymerase - easier and faster optimization. It is also useful for multiplex reactions. In special applications Stoffel Fragment has proven better specificity than regular Taq polymerase. It is espacially recomended for amplifications of small fragments from gDNA. The absence of the 5'-3' exonuclease activity makes it very suitable for cycle sequencing. It gives higher sequence intensity and low backgrounds.
