Guanidine thiocyanate (CAS: 593-84-0) is an ultrapure chaotropic reagent and commonly utilised for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nuclei and is... Read More
Guanidine thiocyanate (CAS: 593-84-0) is an ultrapure chaotropic reagent and commonly utilised for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nuclei and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. In solution, it is known as guanidinium thiocyanate.
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