For ultrapure, transfection-grade plasmid DNA isolation im medium scale (50-300 ml of bacterial culture); yield: 200-600 µg DNA from 100 ml culture; isolation time: 120-130 minutes (with DNA precipitation); centrifugation steps: 6000 x g (no need to have ultracentrifuge); lyophilized RNase A included; each column contain special modificated anion-exchange resin.
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