For ultrapure, transfection-grade plasmid DNA isolation im large scale (200-1000 ml of bacterial culture); yield: 1-1.5 mg DNA from 400 ml culture; isolation time: 140-150 minutes (with DNA precipitation); centrifugation steps: 6000 x g (no need to have ultracentrifuge); lyophilized RNase A included; each column contain special modificated anion-exchange resin and is packed separately in foil bag; DNA Elution volume: 12 ml of PMdE Buffer by gravity flow.