A convenient reaction mixture for fast and reliable quantitative Real-Time PCR using probes, including TaqMan®, Scorpions® and molecular beacon probes. Created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. Used in probe based Real-Time PCR assays, including singleplex and multiplex gene expression studies. For the commodity in performance the set contains ROX passive dye in two concentrations 50x High ROX solution, 50x Low ROX solution.
