Adazol Total RNA Extraction Reagent, 1 pack=100mL
Catalogue Number BAL110
Synonym(s):
Product Application:
Suitable for total RNA extraction from animal/plant cells, tissues, yeast, and bacteria. The purified RNA is ideal for molecular biology applications including RT-PCR and Northern blot analysis.
Instructions for Use:
Using the guanidinium isothio... Read More
- Commodity Code
3824999699
- Chemical Hazard
- TBC
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Product Description
Product Application:
Suitable for total RNA extraction from animal/plant cells, tissues, yeast, and bacteria. The purified RNA is ideal for molecular biology applications including RT-PCR and Northern blot analysis.
Instructions for Use:
Using the guanidinium isothiocyanate/phenol/chloroform method - First add the reagent to lyse the sample then add chloroform to separate the liquid phases, take the upper aqueous phase to precipitate and recover RNA with isopropanol, wash with ethanol, air-dry and then dissolve with DEPC water or TE buffer.
Amount to Use:
For 50-100mg of tissue, adherent cells with a culture area of 10cm², 5-10×10⁶ animal or plant cells or yeast cells, or 1×10⁹ bacterial cells, 1mL of reagent is required. For each 1mL of reagent, 0.2mL of chloroform, 0.5mL of isopropanol and 1mL of 75% ethanol should be added.