TMB Ready-To-Use chromogenic substrate contains 3,3',5,5'- tetramethylbenzidine and H202 in a Citric Acid buffer and is recommended for use in ELISA procedures.
TMB is a substrate for horseradish peroxidase. It produces a soluble blue color product that can be read spectrophotometrically at 370 or 620-650 nm. The reaction may be amplified threefold when stopp
ed with acid, generating a yellow color with its absorption maximum at 450nm. After stopping, it should be read within 30 minutes.
Not recommended for use in immunohistochemical or blotting assays. Stability: Minimum of 1 year if stored at 2-8°C.
H315: Causes skin irritation.
H319: Causes serious eye irritation.
H335: May cause respiratory irritation.
Precautionary Statements
P261: Avoid breathing <dust/fumes/gas/mist/vapours/spray>.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear <protective gloves/protective clothing/eye protection/face protection>.: hazard.P261: Avoid breathing <dust/fumes/gas/mist/vapours/spray>.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear <protective gloves/protective clothing/eye protection/face protection>.